By focusing on nephrology and oxidative stress, Ethos Biosciences Exocell ELISA Kits provide the right tools for your kidney disease research.
PRECISION ELISAS FOR YOUR NEXT DISCOVERY
ELISA, an acronym for enzyme-linked immunosorbent assay, is a well-established microtiter or 96-well plate-based method for determining the concentration of a particular biomarker such as protein, hormone, peptide, antibody, or other biomolecules from samples such as urine, blood, or cell culture medium. ELISAs can be direct, indirect, sandwich, or competitive formats (Figure 1) for quantifying the amount of analyte using color-based reaction that is compared to a standard curve.
Click image to expand
Figure 1. ELISA formats. Enzyme-linked immunosorbent assays (ELISAs) have different capture mechanisms (sandwich, direct, and competitive) and different detection formats (direct or indirect). For sandwich captures, two independent antibodies must recognize two different epitopes of the antigen/analyte, therefore, this format is for larger analytes. Direct capture, in contrast, is usually for smaller analytes. Competitive ELISAs have antigens/analytes attached to the surface of the microwell plate. If no antigens/analytes are in the sample, the added detection antibodies bind to the plate surface. If the sample contains antigen/analytes, they compete with the plate-bound antigen/analytes and reduce the final signal. For direct detection, horseradish peroxidase (HRP) is attached to the detection antibody. For antigens/analytes with low abundance, the final color signal can be enhanced using indirect detections because two secondary antibodies can bind to one primary antibody, resulting in double the HRP molecules for color production.
Ethos Biosciences ELISA Kits & Oxidative Stress Assays
| Analytes | Human | Mouse | Rat |
|---|---|---|---|
| Guca2b | Guca2b ELISA Kit RUO | NA | NA |
| Albumin | Albuwell Hu | Albuwell M | Nephrat II |
| Creatinine | Creatinine Companion | Creatinine Companion | Creatinine Companion |
| Nephrin | Human Nephrin ELISA | Mouse Nephrin ELISA | Rat Nephrin ELISA |
| Cystatin C | Human Cystatin C ELISA | Mouse Cystatin C ELISA | Rat Cystatin C ELISA |
| NGAL | Human NGAL ELISA | Mouse NGAL ELISA | Rat NGAL ELISA |
| Oxidative Stress Assays | TBARS Malondialdehyde Oxidative Stress | DTNB (Ellman’s) Thiol Oxidative Stress |
Discover more ELISA resources: