What is albumin?
Albumin is a protein that carries vitamins, enzymes, and hormones throughout your body. Albumin is made by your liver & it prevents fluid from leaking into your bloodstream. Low albumin levels points to issues with your kidneys or liver.
Why choose Exocells Albumin ELISAs?
Our Albumin ELISAs efficiently assess kidney function through ELISA-based microalbuminuria measurement from human, mouse, sheep, and rat samples. Part of our Exocell ELISA portfolio, each Albumin ELISA kit provides robust quantitative detection and monitoring of microalbuminuria, which is defined as concentrations of albumin in the urine that are not detectable by conventional dipstick methods. These Albumin ELISA assays are highly specific, easy to use, and assist in monitoring the development of incipient nephropathy.
Unlike other Albumin ELISA kits which are designed to measure the much higher concentrations of albumin present in plasma or serum, Albuwell and Nephrat II ELISAs are optimized for highly accurate and precise measurement of the much lower concentrations of albumin present in the urine during nephropathy or kidney injury. Included reagents are either ready-to-use or require minimal dilution, which limits the introduction of variability. Figure 1 demonstrates the robustness of the Albuwell M assay during generation of a standard curve, where the average coefficient of variation (CV) is only 4.166% (n = 8).
Albumin ELISA Technical Details
Albuwell H, M, O, and Nephrat II ELISAs are set up as competitive ELISA assays. The human, mouse, sheep, and rat kits use direct detection for the most streamlined workflows, with the sheep kit implementing indirect detection using rabbit anti-ovine albumin as the primary antibody. Detection is accomplished via conjugated horseradish peroxidase (HRP) and a colorimetric substrate that absorbs at 450 nm.
Our Albumin ELISA Kits
Albuwell Hu (Human Albumin ELISA)
Product #: 1004
Specimen Required: 10 μl, 50 μl, or 100 μl
Assay Range: 0.15-2.560 μg/ml
Precision: Intra and interassay precision for samples within the assay range have a C.V. <7%
Specificity: The antibody is specific for human albumin. It does not cross-react with rat albumin or with other proteins that occur in urine.
Albuwell M (Mouse Albumin ELISA)
Product #: 1011
Specimen Required: Urine, 10 μL
Assay Range: 0.156-10 μg/ml
Precision: Intrassay and interassay precision for samples within useful range of the assay have a C.V. < 10% of the mean.
Nephrat II (Rat Albumin ELISA)
Product #: NR002
Specimen Required: Urine, 10 μl
Assay Range: 2.0-100 μg/ml
Precision: Intrassay and interassay precision for samples within the useful range have a C.V. <10%.
Specificity: No cross-reactivity with human, sheep, rabbit, or bovine albumin. Negligible cross-reactivity with mouse albumin.