Nephrology Assay

Efficiently assess glomerular integrity in studies of diabetes, renal disease, and other pathogenic processes with our ready-to-use ELISA kits.

FOR RESEARCH USE ONLY

Used as a biomarker for kidney injury and dysfunction, urinary nephrin levels are an important research tool for studying renal diseases and diabetes. Advance these studies with our Nephrin ELISA Kits, optimized for nephrin detection in human and rat urine samples. Part of our reliable Exocell ELISA portfolio, both human and rat kits are configured as competitive ELISAs for specific and robust detection without the need for extensive sample pre-processing. Detection is using an indirect method, with a primary antibody raised in mouse against the N-terminal portion of the nephrin protein from either rats (for the rat ELISA) or humans (for the human ELISA), and an anti-mouse secondary antibody conjugated to horseradish peroxidase (HRP). Measurement is accomplished via a colorimetric substrate that absorbs at 450 nm (note that because these are competitive assays, increasing nephrin levels result in decreasing signal).