Rat Nephrin ELISA
Product #: 1036
Specimen Required: Urine, 100 μl
Assay Range: 0.031-2.0 μg/ml
Precision: Intrassay and interassay precision for samples within the useful range have a C.V. <10%
Specificity: Rabbit anti-nephrin antibody reacts with several mammalian species including rodent and human
Rat Nephrin ELISA
(r Nephrin ELISA, Strip Plate) – For Research Use Only
The Rat Nephrin ELISA kit contains all of the components and reagents you need to assay rat nephrin in up to 40 duplicate urine or cell free liquid samples (plasma, serum, cell culture supernatant, etc…).
Developed by the nephrology experts at Exocell, the Rat Nephrin ELISA kit has been newly updated to remove any cross reactivity to albumin. Nephrin is a transmembrane protein expressed in renal glomerular podocytes; studies in experimental animals and human diabetes suggest nephrinuria is a marker for glomerular filtration dysfunction or podocyte injury.
As an indirect competitive ELISA, Rat Nephrin uses an HRP labeled antibody to detect minute levels of nephrin, primarily in urine. The Rat Nephrin ELISA contains 1 x 96 well plate, and accounting for standard wells, 80 samples or 40 samples in duplicate (recommended) can be analyzed using one kit. Overnight incubation allows users to prepare the assay on the first day and assess the data on the second day.
Kit Contents include:
- One Rat Nephrin Assay Plate
- Two EIA Diluent
- One Rat Nephrin Standard
- One Anti-rat Nephrin Antibody
- One Anti-mouse IgG-HRP Conjugate
- One Color Developer/Stopper
Rat Nephrin ELISA Citations
Turbeville, Taylor, Garrett et al
Hypertension (2019) 73 (3), 650-658 DOI: 10.1161/HYPERTENSIONAHA.118.12097
Katary, Pye, Elmarakby
Prostaglandins Other Lipid Mediat. (2017) 132, 3-11 DOI: 10.1016/j.prostaglandins.2016.08.004
|Dimensions||11 × 10 × 15 in|