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Human Cystatin C ELISA

1041


Human Cystatin C ELISA: Competitive assay for the quantitative determination of human Cystatin C

Intended Use: Human Cystatin C ELISA is designed to measure human cystatin C in a competitive format in urine, serum, saliva, or cell culture supernatant. It is for research purposes and is not intended for diagnostic use.

Description: Human Cystatin C ELISA uses recombinant human cystatin C (Ser27-Ala146) as standard and a polyclonal antibody raised against recombinant cystatin C. This antibody does not react with human albumin or human IgG either in this ELISA or on Western Blot. Natural cystatin C showed dose-response curves that were parallel to the standard curves obtained using the Ethos Biosciences kit standards, indicating that this kit can be used to determine relative levels of native cystatin C. The assay is conducted in an indirect competitive mode and the results are reported as ng/ml human cystatin C.

The Human Cystatin C ELISA plates are coated with cystatin C and are supplied blocked and dried. The plates must be washed 2 times before use with wash buffer. To complete the assay, the diluted standards and samples are added to respective wells. The anti-cystatin C antibody is then added. This antibody interacts and binds with the human cystatin C in solution or with the cystatin C immobilized to the stationary phase, hence the notion of competitive binding. A subsequent reaction with HRP-conjugated second antibody then binds to the anti-cystatin C antibody bound to the stationary phase of the plate. The bound conjugated antibody is then detected using a chromogenic reaction. Color intensity in Human Cystatin C ELISA is inversely proportional to the logarithm of the concentration of human cystatin C in the fluid phase.

Kit Contents:

  • 1 x 96-well Cystatin C Assay Plate
  • 2 x NHE-Casein Diluent
  • 1 x Human Cystatin C Standard
  • 1 x Anti-Cystatin C Antibody
  • 1 x HRP-Conjugate Antibody
  • 1 x TMB Color Developer
  • 1 x Acid Color Stopper
  • 1 x Instruction

Performance Characteristics:

Average CV% <8%
Analytical Range 8-500 ng/ml
Dose Response 1.5 OD
Model Semi-log
R² >0.97
Linearity Yes
Spike/Recovery Yes
Stability/Shelf Life Real time
Sample Validation Yes
Normal Value Urine 30-300 ng/ml
Normal Value Plasma 0.6-1.2 ug/ml

 

Standard Curve:


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