How to Use the H. pylori Quick III Kit: A Complete Guide for Histology Applications

Helicobacter pylori (H. pylori) is a spiral-shaped bacterium associated with chronic gastritis, peptic ulcers, and gastric cancer. Ethos Biosciences offers a fast, reliable solution with the Quick III Kit – a three-step staining system.

In this article, we’ll walk through 1 optimized procedure for using the Quick III Kit: Histology slides, ensuring consistent and professional results across lab environments

What Is the Quick III Kit?

The Quick III Kit includes:

• Quick III Fixative – Preserves cellular structure.
• Quick III Solution I (Eosin) – Stains cytoplasmic components.
• Quick III Solution II (Thiazine) – Highlights nuclei and microbial organisms.

These ready-to-use, certified dyes are designed for rapid staining, delivering results comparable to Wright-Giemsa protocols in under a minute.

Procedure : Histology – Paraffin-Embedded Slides

This method demonstrates H. pylori presence in tissue biopsies using paraffin-embedded slides.

Step-by-Step Instructions

1. Deparaffinization
   Pass the slide through xylene, then graded alcohols, ending in distilled water.
2. Solution I (Orange – Eosin)
   Place the slide in Solution I for 1 minute.
3. Solution II (Blue – Thiazine)
   Transfer directly into Solution II for 1 minute.
4. Quick Rinse
   Rinse gently to remove excess stain.
5. Acetic Acid Dip
   Briefly dip in 0.25% acetic acid to enhance differentiation.
6. Alcohol Differentiation
   Pass through 95% alcohol, then absolute alcohols.

Clearing & Mounting
Clear in xylene, then apply a coverslip as usual.

Best Practices for Consistent Results

• Use fresh reagents and clean containers.
• Follow timing precisely for optimal staining.
• Avoid cross-contamination between solutions.
• Document findings with clinical context.

Why Choose Ethos Biosciences?

Ethos Biosciences delivers:

• Speed – Results in under 60 seconds.
• Accuracy – High-quality, certified dyes.
• Versatility – Works on blood, microbes, and tissue sections.
• Reliability – Trusted by clinical labs and researchers.

Conclusion

The Quick III Kit from Ethos Biosciences offers a fast, professional-grade solution for rapid H. pylori testing with tissue samples. By following these procedures, labs can ensure consistent, high-quality results that support better diagnostics and patient care.

Want to try it yourself? Request a sample here.

Need more help? View our Resources page or contact our Technical Experts with any of your questions at: Info@ethosbiosciences.com or 1.800.441.0366. We’re always happy to help you!