Human NGAL ELISA
Product #: 1038
Specimen Required: Urine, 100 μl
Assay Range: 0.495-1.886 μg/ml
Precision: Intrassay and interassay precision for samples within the useful range have a C.V. <10%
Intended Use: Ethos Biosciences’ Human NGAL ELISA is designed to measure human NGAL in biological samples in a direct, antibody sandwich format. It is for research purposes, and is not intended for diagnostic use.
Description: Ethos’ Human NGAL ELISA uses recombinant human NGAL as standard and two antibodies raised against recombinant human NGAL. The antibodies do not react with human albumin or human IgG. Human urine samples showed dose-response curves that were parallel to the standard curve indicating that this kit can be used to determine relative levels of native human NGAL. The assay results are reported as ng/ml human NGAL.
The Ethos Human NGAL ELISA plate is supplied coated, blocked, dried and ready to use. To complete the assay, the plate is washed 2 times and diluted standards and samples are added to respective wells and the plate incubated. After washing, the anti-human NGAL-HRP conjugate is added. This antibody interacts and binds with the NGAL bound to the antibody coated on the plate. The bound conjugated antibody is then detected using a chromogenic reaction. Color intensity in Ethos’ Human NGAL ELISA is proportional to the concentration of human NGAL in the sample.
- 1 x 96-well Human NGAL Assay Plate
- 2 x NHE-BSA Diluent
- 1 x Human NGAL Standard
- 1 x Anti-Human NGAL-HRP Conjugate
- 1 x TMB Color Developer
- 1 x Acid Color Stopper
- 1 x Instruction Manual
All kit reagents are supplied in ready to use liquid form.
|6 × 6 × 6 in