Verhoeff Van Gieson Elastin Stain Kit

Description

The Verhoeff Van Gieson Elastin stain is useful in demonstrating atrophy of elastic tissue and the thinning and loss of elastic fibers. Tissue is stained with a regressive hematoxylin consisting of ferric chloride and iodine. Ferric chloride and iodine act as mordants and aid in the oxidation of hematoxylin to hematin, which is responsible for staining elastic elements. The differentiating is accomplished by using excess mordant, ferric chloride, to break the tissue-mordant dye complex. The dye will be attracted to the larger amount of mordant in the differentiating solution and is removed from the tissue. The elastic tissue has the strongest affinity of the iron-hematoxylin complex and will hold onto the dye longer than the other tissue elements. Sodium thiosulfate is used to remove any excess iodine.

Kit contains: Solution A – 5% Alcoholic Hematoxylin, Solution B – 10% Aqueous Ferric Chloride, Solution C – Iodine Solution, Solution D – 5% Sodium Thiosulphate, Solution E – Counterstain I, Solution F – Counterstain II. 500 ml kit stains up to 120 slides.