For the measurement of rat urine albumin.
Intended Use: Nephrat is an enzyme linked immunosorbent assay (ELISA) for the quantitative determination of albumin in rat urine. It is for research purposes, and is not intended for in vitro diagnostic use.
Kit Contents: Your Nephrat kit should contain the following items:
a. 2 Assay Plates
b. 2 NHEBSA Diluent
c. 1 Rat Serum Albumin (RSA) Standard
d. 2 Nephrat Conjugate.
e. 2 TMB Color Developer
f. 2 Acid Color Stopper
Technical Background: Nephrat is used as a research tool for assessing kidney function in rats. It is simple to perform and highly specific for rat albumin. It is a conventional direct competitive ELISA that recognizes antigen (albumin) in test samples.
To complete the assay, sample and anti-rat albumin antibody-HRP conjugate are added to an albumin-coated well. The antibody binds to the albumin immobilized onto the stationary phase or to the albumin in the fluid phase, hence the notion of competitive binding. After washing, only the antibody-conjugate that has bound to the albumin of the stationary phase will remain in the well, and this is detected using a chromogenic reaction. Color intensity in Nephrat is inversely proportional to the logarithm of albumin concentration in the fluid phase.
Specimen Collection and Storage: Collect samples without preservative, and clarify them by centrifugation if necessary. Store clarified urine at 4C for up to 1 week or at -60C for up to 2 months. Prior to assay, allow the samples to come to room temperature. Do not apply heat to thaw frozen samples.
RSA Standard, NHEBSA Diluent, anti-Rat Albumin Antibody HRP preparations contain 0.05% Proclin 300 (active components isothiazolones) as a preservative. Acid Color Stopper contains dilute (2.0 N) sulfuric acid.
Nephrat Assay Plates are precoated and ready to use. All kit reagents are supplied in ready-to-use liquid form. A provision to wash the plates should be made, tap water may be used, but an EIA Wash Buffer with composition: 0.15 M NaCl, 0.01 M triethanolamine (pH 6.8),
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