(m Beta-induced h3 ELISA, Strip Plate)
The Beta-IG-H3 kit contains all of the components and reagents you need to assay mouse beta-induced h3 protein in up to 40 duplicate urine or cell free liquid samples (plasma, serum, cell culture supernatant, etc…).Beta-induced h3 protein is synonymous with transforming growth factor-beta-induced protein ig-h3, TGF-beta Induced Gene H3, and RGD-CAP.
βIG-H3-M ELISA: An ELISA designed to measure βig-h3 in urine, serum or cell culture supernatant of mouse origin.
Intended Use: βIG-H3-M ELISA is a quantitative sandwich ELISA for measurement of mouse βig-h3 found in urine, serum, and supernatant. It is for research purposes, and is not intended for diagnostic use.
Kit Contents: The βIG-H3-M ELISA kit contains the following items:
a. 1 βIG-H3-M Assay Plate
b. 1 βIG-H3-M Diluent
c. 1 βIG-H3-M Standard
d. 1 Biotinylated sheep anti-βIG-H3-M
e. 1 Streptavidin-HRP Conjugate
f. 1 Color Developer
g. 1 Color Stopper
h. 1 Instructions
Technical Background: βIG-H3 (beta-induced gene h3 ) is a 68 kD protein induced in diverse cell types by Transforming Growth Factor-ß (TGF-ß) that is secreted into the extra-cellular matrix and is involved in cell growth, differentiation, adhesion, and wound healing. ßIG-H3 expression is altered in various conditions including tumorigenesis, breast cancer, corneal dystrophy, osteogenesis, and atherothrombosis. ßig-h3 is elevated in the urine of patients with cyclosporine nephrotoxicity and with type 2 diabetes in whom it shows positive correlation with the albumin excretion rate, making it a useful biomarker of renal dysfunction due to diabetes in its earliest phases and in monitoring clinical progression of diabetic nephropathy.
βIG-H3-M ELISA uses a 96-well assay plate pre-coated with sheep anti-mouse βIG-H3 as capture antibody. Appropriately diluted recombinant mouse βIG-H3 standard and samples bind to the capture antibody. The βIG-H3 is then labeled with a biotinylated polyclonal sheep anti-mouse βIG-H3 antibody. A subsequent reaction with Streptavidin-HRP conjugate labels the biotinylated probe with enzyme.
After washing, only the βIG-H3 bound to the capture antibody on the stationary phase will be detected using a chromogenic reaction. Color intensity in βIG-H3-M ELISA is proportional to the amount of βIG-H3 captured on the plate.
Specimen Collection and Storage: Collect samples without preservative, separate and clarify them by centrifugation if necessary. Samples may be stored at 4˚C for up to 1 week or at -60˚C for up to 2 months. Prior to assay, allow the samples to come to room temperature. Do not apply heat to thaw frozen samples.
Specimen Required: Urine, serum or tissue extract. (Mouse)
Assay Range:0.156 – 10.0 ng/ml
Precision: Intra- and inter-assay precision for samples within the assay range have a C.V. of <10%.