Human Hemoglobin A1c 1.0 mg

hHbA_1c is prepared from lysed human blood cells by sequential ion-exchange chromatographic methods. Essentially free of HbA_1a, HbA_1b and HbA0_a0, the purified protein is validated by HPLC methods and carbohydrate analysis, and is suitable as a reference material for hHbA_1ctesting. The reported concentration is determined by Drabkins assay.

The material is supplied in liquid form in dilute potassium cyanide-phosphate buffer. The concentration varies with manufacturing lot, but the protein may be used directly by dilution in buffer in most experimental methods. It is shipped frozen at -20^oC.

Short term storage should be done at 4^oC. Long term storage should be completed at -60^oC. Repeated cycles of freezing and thawing should be avoided.

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