Glyco-hemoglobin is the glycated fraction of human hemoglobin A0 prepared from lysed human blood cells by sequential ion-exchange and affinity chromatographic methods. It has 0.5-2.0 glyco groups per hemoglobin molecule, but it is free of HbA1a, HbA1b, HbA1c and HbF. The protein is validated by HPLC methods, and is suitable as a primary standard in chromatographic or immunologic analyses. The reported concentration is determined by Drabkins assay.
The material is supplied in liquid form in dilute potassium cyanide-phosphate buffer. The concentration varies with manufacturing lot, but the protein may be used directly by dilution in buffer in most experimental methods. It is shipped frozen at -20oC
Short term storage should be done at 4oC. Long term storage should be completed at -60oC. Repeated cycles of freezing and thawing should be avoided.
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