Out of stock
(hamster ELISA, Strip plate)
The Albuwell H kit contains all of the components and reagents you need to assay hamster albumin in up to 80 duplicate urine or cell free liquid samples (plasma, serum, cell culture supernatant, etc…).
Intended Use: Albuwell H is an enzyme linked immunosorbent assay (ELISA) for the quantitative determination of albumin in hamster urine. It is for research purposes, and is not intended for diagnostic use.
Kit Contents: Your Albuwell H kit should contain the following items:
- 2 Albuwell H EIA Plates: Coated with hamster albumin
- 2 NHEBSA Diluent
- 1 Hamster Serum Albumin
- 2 Rabbit Anti-hamster Albumin
- 2 Anti-rabbit (IgG) HRP Conjugate
- 2 Color Developer
- 2 Color Stopper
Hamster Albumin Standard, NHEB Diluent, Rabbit anti-hamster Albumin Antibody and Anti-rabbit HRP Conjugate preparations contain 0.05% Proclin 300 (active components isothiazolones) as preservative. Color Stopper contains dilute (2.0 N) sulfuric acid.
Albuwell H plates are precoated and ready to use. All kit reagents are supplied in ready to use liquid form. A provision to wash the plates should be made, and tap water is usually satisfactory. Where tap water is contaminated, an EIA Wash Buffer with composition: 0.15 M NaCl, 0.01 M triethanolamine (pH 6.8), 0.05% Tween 20 and 0.05 % Proclin 300 (preservative may be omitted if the buffer is freshly prepared) is recommended for washing the plate.
Micropipettors capable of delivering 10, 50, 100 and 200 uL are required. Multi-channel pipettors capable of delivering 50 and 100 uL are recommended. In addition, small test tubes are required to complete dilutions (microfuge tubes work well in this application). Finally, a microplate reader equipped to determine absorbance at 450 nm is required.
Technical Background: Albuwell H is for in vitro use as a research tool for assessing kidney function in hamsters. It is a conventional indirect competitive ELISA that recognizes antigen (albumin) in test samples.
To complete the assay, sample and rabbit anti-hamster albumin antibody are added to an albumin-coated well. The antibody interacts and binds with the albumin immobilized to the stationary phase or with that in the fluid phase, hence the notion of competitive binding. After washing, a
subsequent reaction with anti-rabbit (IgG) HRP conjugate labels the probe with enzyme. A second washing leaves only the antibodyconjugate that bound to the albumin of the stationary phase in the well, and this is detected using a chromogenic reaction. Color intensity in Albuwell H is inversely porportional to the logarithm of albumin in the fluid phase.
Specimen Collection and Storage: Collect samples without preservative, and clarify them by centrifugation if necessary. Store clarified urine at 4oC for up to 1 week or at 60oC for up to 2 months. Prior to assay, allow the samples to come to room temperature. Do not apply heat to thaw frozen samples.