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Track cardiac muscle damage in animal models of disease and other research studies using Exocell Troponin I ELISA Kits.
FOR RESEARCH USE ONLY
Exocell Troponin I ELISA Kits support a range of studies with high-quality measurement of troponin I levels in serum, plasma, or cell culture supernatants. Available for samples from rat, mouse, or humans, these kits are configured as quantitative sandwich ELISAs that use indirect detection via a secondary antibody conjugated to horse radish peroxidase (HRP). Measurement uses a colorimetric HRP substrate that absorbs at 450 nm and color intensity in all Troponin I ELISAs is proportional to the amount of troponin I captured on the plate.
While Exocell Troponin I ELISAs can be used for any application where accurate and precise measurement of troponin I is needed, researchers using other Exocell ELISAs to monitor diabetic nephropathy as well as cardiac damage can ensure study consistency and quality by also choosing an Exocell Troponin I ELISA.
As with all of the Exocell ELISA products, our Troponin I ELISA Kits deliver the same reliable results and expert support that Exocell customers know to expect, including reagents that are ready to use either as is or with only moderate dilution.
What’s a sandwich ELISA?
In a sandwich ELISA, antigen measurement occurs by building an antibody-antigen-antibody “sandwich.” First, antibody that recognizes the target antigen is immobilized in the wells of a multi-well plate. The sample is added to the plate and target antigen binds to the immobilized antibody. After washing, a solution containing soluble antibody that recognizes the target antigen is added to detect and measure the amount of target antigen present in the wells.
To make a sandwich ELISA quantitative, it is critical to achieve uniform and consistent coating of the initial antibody to the multi-well plate.
Why use a sandwich ELISA?
Sandwich ELISAs are useful when purified target antigen is not available to create a competitive ELISA plate or when antibody to the target antigen is plentiful.
Troponin I is a component of the troponin complex, a group of three proteins—troponin C, troponin I, and troponin T—which are involved in the biomechanical regulation of skeletal and cardiac muscle contraction. Because myocardial damage releases troponin I and T into the bloodstream, blood levels of both proteins are sensitive and specific indicators of myocardial damage and can indicate the extent of injury to the heart in response to disease or drug-induced cardiotoxicity. In addition, cardiac troponins may aid in predicting cardiovascular risk and mortality in the presence of chronic renal disease.
Human troponin I has three separate isoforms—two in skeletal muscle fibers and one in cardiac muscle. The cardiac isoform (cTnI) has a molecular weight of 22.5 kDa and is about 40% different from the skeletal isoforms. Antibodies specific for troponin I do not react immunologically with the other skeletal isoforms and are unique for the cardiac isoform.