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Soluble Tumor Necrosis Factor Receptor 2 (sTNFR2)

Study the development of chronic kidney disease and other disorders that use soluble tumor necrosis factor receptor 2 as a biomarker.

FOR RESEARCH USE ONLY

Our Exocell sTNFR2 ELISA Kit supports a range of studies with high-quality measurement of human sTNFR2 levels in serum, plasma, or cell culture supernatants. The sTNFR2 Kit is configured as a quantitative sandwich ELISAs that use biotinylated detection antibodies. Measurement occurs through streptavidin-conjugated horse radish peroxidase (HRP) which binds to the biotinylated antibody, and a colorimetric HRP substrate that absorbs at 450 nm. Color intensity in all sTNFR2 ELISAs is proportional to the amount of sTNFR2 captured on the plate.

 

Why choose the Exocell sTNFR2 ELISA?

A biomarker for inflammation, measurement of circulating sTNFR2 levels can inform studies of inflammation-related disorders including chronic kidney disease. Ensure the quality and consistency of your results by choosing an ELISA prepared by the same team of scientists that make your other Exocell products.

As with all of the Exocell ELISA products, our sTNFR2 ELISA Kit delivers the same reliable results and expert support that Exocell customers know to expect, including reagents that are ready-to-use either as is or with only moderate dilution.

Kit Contents:

  1. 1 TNF-sR2 Assay Plate
  2. 2 TNF-sR2 Diluent
  3. 1 TNF-sR2 Standard
  4. 1 Biotinylated goat anti-TNF-sR2
  5. 1 TNF sR2 Diluent with NGS
  6. 1 Streptavidin-HRP Conjugate
  7. 1 Color Developer
  8. 1 Color Stopper
  9. 1 Instructions

Background: TNFR2 is a 75 kDa cell surface receptor for TNFα, and initiates both classic and alternative NFκB signaling pathways1. Cells expressing TNFR2 can shed the extracellular domain into the bloodstream, resulting in soluble TNFR2 (also referred to as circulating TNFR2) which attenuates TNFα signaling through competitive binding.

Circulating TNFR2 is associated with cardiovascular and renal disease, and particularly with the development of chronic kidney disease in diabetes.

Specimen Collection and Storage: Collect samples without preservative. Separate and clarify samples using centrifugation, if necessary.

Samples may be stored at 4˚C for up to 1 week or at -60˚C for up to 2 months. Prior to assay, allow the samples to come to room temperature. Do not apply heat to thaw frozen samples.

Compatible specimens: Tissue culture supernatant, urine, serum or plasma (human). 

Assay Range: 15.6 to 1000 pg/ml

Precision: Intra- and inter-assay precision for samples within the assay range have a C.V. of <10%.

REFERENCES

  1. Wajant H and Siegmund D. TNFR1 and TNFR2 in the Control of the Life and Death Balance of Macrophages. Cell Dev. Biol., 29 May 2019 | https://doi.org/10.3389/fcell.2019.00091