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Malondialdehyde Oxidative Stress Assay

1020


Previously known as Universal TBARS Assay

The Malondialdehyde Oxidative Stress Assay is a tool for the direct quantitative measurement of MDA in biological samples formed from lipid peroxidation due to oxidative stress. Oxidative modification of lipids occurs with aging and various diseases, especially diabetes and kidney disease. Because lipid peroxidation is a complicated process, we recommended that multiple oxidative stress assays be used in conjunction, such as Exocell's Thiol Oxidative Stress Assay

Our kit uses thiobarbituric acid which forms an adduct with MDA. The samples are first reacted with TBA at 75˚C. After the overnight incubation the standards and samples are read either fluorometrically or spectrophotometrically. The MDA content in the samples is determined by linear regression comparison of the standard curve. The sensitivity of measuring Thiobarbituric Acid Reactive Substances (TBARS) has made this assay the method of choice for screening and monitoring lipid peroxidation.

The Malondialdehyde Oxidative Stress Assay kit contains all of the components and reagents you need to conduct a fluorescence assay for MDA of any animal species in up to 89 single urine or cell free liquid samples (plasma, serum, cell culture supernatant, etc…). Directions for an optical assay are included and may be conducted by using your own clear plate.

Materials Supplied In Kit:
Thiobarbituric Acid stock solution
Assay Diluent
MDA Standard (10 uM MalondialdehydeBis)
Acetic Acid
Microplate (1) for fluorometer

Materials required but not supplied:
Eppendorf tubes
Water bath or heat block set at 75˚
Adjustable pipettes
Graduated cylinders and dilution test tubes
Disposable gloves
96 well fluorometer for reading
Distilled H2O
Spectrophotometer for reading colorimetrically
Protein Assay kit if doing ratio to protein 

Storage: Store all kit reagents at 2-8˚C. The components should be used before the expiration date indicated on the outside of the box. TBA stock
should be refrigerated.


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