(h tumor necrosis factor soluble receptor II ELISA)
TNF-sR2 ELISA: An ELISA designed to measure Tumor Necrosis Factor Soluble Receptor II of human origin in plasma, serum, urine or cell culture supernatant.
Intended Use: TNF-sR2 ELISA is a quantitative sandwich ELISA for
measurement of humanTNF-sRII found in plasma, serum, and
supernatant. It is for research purposes, and is not intended for
Kit Contents: The TNF-sR2 ELISA kit contains the following items:
a. 1 TNF-sR2 Assay Plate
b. 2 TNF-sR2 Diluent
c. 1 TNF-sR2 Standard
d. 1 Biotinylated goat anti-TNF-sR2
e. 1 TNF sR2 Diluent with NGS
f. 1 Streptavidin-HRP Conjugate
g. 1 Color Developer
h. 1 Color Stopper
i. 1 Instructions
Technical Background: TNF-sR2 ELISA measures the soluble receptor
II of TNF α in urine, plasma, serum or cell culture supernatant of human
origin. The 55-kD TNFαR1 and 75-kD TNF αR2 are specific cell surface
receptors for TNF α, an inflammatorymediating cytokine, that elicits TNFmediated
responses through activation of cell signaling pathways. TNF α and its receptors are shed from the cell surface, with TNF α appearing in the plasma as free
cytokine or bound to circulating receptors released into the extracellular space. Circulating concentrations of TNF αRII have been found to be associated with
cardiovascular and renal disease, and particularly with the development
of chronic kidney disease in diabetes. Specimen Collection and Storage:
Collect samples without preservative, separate and clarify them by centrifugation if necessary.
Samples may be stored at 4˚C for up to 1 week or at -60˚C for up to 2 months. Prior to assay, allow the samples to come to room temperature. Do not apply
heat to thaw frozen samples.
Specimen Required: Tissue culture supernatant, urine, serum or
plasma (human). Assay Range:15.6 to 1000 pg/ml
Precision: Intra- and inter-assay precision for samples within the assay range have a C.V. of <10%.
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