(h Beta-induced h3 ELISA, Strip Plate)
The Beta-IG-H3 kit contains all of the components and reagents you need to assay human beta-induced h3 protein in up to 40 duplicate urine or cell free liquid samples (plasma, serum, cell culture supernatant, etc…).Beta-induced h3 protein is synonymous with transforming growth factor-beta-induced protein ig-h3, TGF-beta Induced Gene H3, and RGD-CAP.
Intended Use: βIG-H3-H ELISA is a quantitative sandwich ELISA for measurement of human βig-h3 found in urine, serum, and supernatant. It is for research purposes, and is not intended for diagnostic use.
Kit Contents: The βIG-H3-H ELISA kit contains the following items:
a. 1 βIG-H3-H Assay Plate
b. 1 βIG-H3-H Diluent
c. 1 βIG-H3-H Standard
d. 1 Biotinylated goat anti-βIG-H3-H
e. 1 Streptavidin-HRP Conjugate
f. 1 Color Developer
g. 1 Color Stopper
h. 1 Instructions
Technical Background: βig-h3 (beta-induced gene h3 ) is a 68 kD protein induced in diverse cell types by Transforming Growth Factor-ß (TGF-ß) that is secreted into the extra-cellular matrix and is involved in cell growth, differentiation, adhesion, and wound healing. ßig-h3 expression is altered in various conditions including tumorigenesis, breast cancer, corneal dystrophy, osteogenesis, and atherothrombosis. ßig-h3 is elevated in the urine of patients with cyclosporine nephrotoxicity and with type 2 diabetes in whom it shows positive correlation with the albumin excretion rate, making it a useful biomarker of renal dysfunction due to diabetes in its earliest phases and in monitoring clinical progression of diabetic nephropathy.
βIG-H3-H ELISA uses a 96-well assay plate pre-coated with mouse anti-human βig-h3 as capture antibody. Appropriately diluted recombinant human βig-h3 standard and samples bind to the capture antibody. The βig-h3 is then labeled with a biotinylated polyclonal goat anti-human βig-h3 antibody. A subsequent reaction with Streptavidin-HRP conjugate labels the biotinylated probe with enzyme.
After washing, only the βig-h3 bound to the capture antibody on the stationary phase will be detected using a chromogenic reaction. Color intensity in βIG-H3-H ELISA is proportional to the amount of βig-h3 captured on the plate.
Specimen Collection and Storage: Collect samples without preservative, separate and clarify them by centrifugation if necessary. Samples may be stored at 4˚C for up to 1 week or at -60˚C for up to 2 months. Prior to assay, allow the samples to come to room temperature. Do not apply heat to thaw frozen samples.
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