The DTNB (Ellman's) Thiol Oxidative Stress Assay Kit is designed to measure free thiol groups that...
Reliably and reproducibly measure biomarkers for oxidative stress with Exocell ELISA Kits, the same experts you trust for nephrology ELISAs.
FOR RESEARCH USE ONLY
Oxidative stress and kidney diseases are often co-morbid in the same patient, especially in aging and diabetic populations. For researchers who want to measure biomarkers for oxidative stress with the same levels of accuracy and reliability as their Exocell nephrology ELISAs, we offer ELISA Kits that measure a number of different analytes that report on oxidative stress. Like all of the Exocell ELISAs, the ELISAs for cardiology research are designed to maximize accurate and precise quantitation, with reagents provided at either ready-to-use or with minimal dilution required to limit the introduction of variability. As a result, you should expect excellent coefficients of variation (CVs) for reliable and reproducible results.
Exocell βig-H3/TGFBI/BIGH3 ELISA Kit:
Exocell Malondialdehyde Oxidative Stress Assay (Universal TBARS Assay)
What do accuracy and precision mean in quantitative measurements?
While accuracy and precision are commonly used in everyday speech, they have very specific meanings when it comes to quantitative measurements. It’s easy to get the scientific definitions confused as they are often used interchangeably in casual conversation. Here’s a quick refresher on the terminology:
How can I ensure accuracy and precision in my ELISAs?
Like all scientists, you want to be able to trust that the data you generate is a true reflection of the biology you are measuring—in other words, you want it to be both accurate and precise.
When it comes to ELISAs, accuracy will primarily depend on the quality of your antibody. Not only should you match the species of your sample to the species of the target analyte used to generate the ELISA’s detection antibody, but you want to be sure that the antibody is highly specific for the target analyte with very low off-target binding. It’s also important to make sure that validation of antibody specificity and sensitivity is performed using an ELISA rather than another type of assay like a western blot or immunoprecipitation, as binding reactions are highly dependent on conditions such as concentration of target and analyte, buffers, etc.
At Ethos, all of our ELISA kits use antibodies that are both sensitive and specific for the target analyte and we validate specificity and sensitivity using the same ELISAs that we sell, so you can have full confidence in your results.
To maximize precision, you need to minimize variability. One way that Ethos helps you minimize variability is by providing ELISA kit reagents that are either ready-to-use or require minimal dilutions. Many ELISA kits come with reagents that might need to be diluted as much as 10,000-fold to measure analytes in urine, but each serial dilution step is a place where variability from pipetting or incomplete mixing can not only appear but get propagated in subsequent dilutions. Thus, limiting the number of dilutions is not only more efficient for your workflow, it’s also better for ensuring precise measurements.