Exocell ELISA Kits for Nephrology Research

Featured papers: See how Exocell ELISAs advance nephrology research

Exocell Collagen IV ELISA Kit:

Comparative efficacy and safety of gemigliptin versus linagliptin in type 2 diabetes patients with renal impairment: A 40‐week extension of the GUARD randomized study

Han SY et al. Diabetes Obes Metab. 2018 Feb;20(2):292-300. doi: 10.1111/dom.13059.

Exocell Nephrin ELISA Kit:

Superimposed Preeclampsia Exacerbates Postpartum Renal Injury Despite Lack of Long-Term Blood Pressure Difference in the Dahl Salt-Sensitive Rat

Turbeville, HR et al. Hypertension. 2019 Mar;73(3):650-658. doi: 10.1161/HYPERTENSIONAHA.118.12097.

Exocell Albuwell M ELISA Kit, The Creatinine Companion:

Disulfide-bond A oxidoreductase-like protein protects against ectopic fat deposition and lipid-related kidney damage in diabetic nephropathy

Chen X et al. Kidney Int. 2019 Apr;95(4):880-895. doi: 10.1016/j.kint.2018.10.038.

Tech Tips

What do accuracy and precision mean in quantitative measurements?

While accuracy and precision are commonly used in everyday speech, they have very specific meanings when it comes to quantitative measurements. It’s easy to get the scientific definitions confused as they are often used interchangeably in casual conversation. Here’s a quick refresher on the terminology:

• Precision—how close a series of replicate measurements are to each other
• Accuracy—how close a measurement is to its “true” value

How can I ensure accuracy and precision in my ELISAs?

Like all scientists, you want to be able to trust that the data you generate is a true reflection of the biology you are measuring—in other words, you want it to be both accurate and precise. 

Ensuring accuracy

When it comes to ELISAs, accuracy will primarily depend on the quality of your antibody. Not only should you match the species of your sample to the species of the target analyte used to generate the ELISA’s detection antibody, but you want to be sure that the antibody is highly specific for the target analyte with very low off-target binding. It’s also important to make sure that validation of antibody specificity and sensitivity is performed using an ELISA rather than another type of assay like a western blot or immunoprecipitation, as binding reactions are highly dependent on conditions such as concentration of target and analyte, buffers, etc. 

At Ethos, all of our ELISA kits use antibodies that are both sensitive and specific for the target analyte and  we validate specificity and sensitivity using the same ELISAs that we sell, so you can have full confidence in your results. 

Ensuring precision

To maximize precision, you need to minimize variability. One way that Ethos helps you minimize variability is by providing ELISA kit reagents that are either ready-to-use or require minimal dilutions. Many ELISA kits come with reagents that might need to be diluted as much as 10,000-fold to measure analytes in urine, but each serial dilution step is a place where variability from pipetting or incomplete mixing can not only appear but get propagated in subsequent dilutions. Thus, limiting the number of dilutions is not only more efficient for your workflow, it’s also better for ensuring precise measurements.