(m Troponin I ELISA, Strip Plate)
The Troponin I kit contains all of the components and reagents you need to assay mouse Troponin I protein in up to 40 duplicate urine or cell free liquid samples (plasma, serum, cell culture supernatant, etc…).
Troponin I M ELISA: An ELISA designed to measure murine Troponin I
in plasma, serum or cell culture supertant.
Intended Use: Troponin I M ELISA is a direct ELISA for quantitation of
murine Troponin I found in serum, plasma and supernatant. It is for
research purposes, and is not intended for diagnostic use.
Kit Contents: The Troponin I M ELISA kit contains the following items:
a. 1 Troponin I Assay Plate
b. 2 NHEBSA Diluent
c. 2 Troponin I M Standard
d. 1 Anti-Troponin I M antibody
e. 1 HRP-conjugated antibody
f. 1 Color Developer
g. 1 Color Stopper
h. 1 Instructions
Technical Background: Troponin I is a complex of three proteins (troponin
C, troponin I, and troponin T) that regulate skeletal and cardiac muscle
contraction. Cardiac troponin I and T are sensitive and specific indicators of
myocardial damage, and their blood levels rise in relation to the extent of
injury to heart muscle from various conditions or from drug-induced
cardiotoxicity. Cardiac troponins may be usefuls in predicting cardiovascular
risk and mortality in the presence of chronic renal disease.
The mouse troponin I has three separate isoforms: two in skeletal
muscle fibers, and one in cardiac muscle. The cardiac isoform (cTnI)
has a molecular weight of 22.5 kdaltons and is about 40% different
from the skeletal isoforms. Antibodies specific for cardiac troponin I do not
react immunologically with the other skeletal isoforms. The Exocell
Troponin I M ELISA is a direct ELISA using two different affinity purified
antibodies to troponin I. The first antibody is coated on a microtitre plate.
The samples are added to the plate and incubated overnight. The plate is
washed and then the anti-troponin I M antibody is added. This reaction is
allowed to incubate for two hours at room temperature. The plate is washed
and the HRP-conjugated antiobody is added for 2 hour. The plate is washed
and the color developer is added for approximately 10 minutes. The murine
Troponin I is then calculated from the standard curve.
Specimen Collection and Storage: Collect samples without preservative,
separate and clarify them by centrifugation if necessary. Samples
may be stored at 4˚C for up to 1 week or at -60˚C for up to 2 months. Prior to
assay, allow the samples to come to room temperature. Do not apply heat
to thaw frozen samples.
Specimen Required: Serum, Plasma or tissue extract.
Assay Range: 0.78 – 50.0 ng/ml
Precision: Intra- and inter-assay precision for samples within the assay
range have a C.V. of <10%.
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