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Mouse Troponin I ELISA

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The Mouse Troponin I kit contains all of the components and reagents you need to assay mouse Troponin I in up to 40 duplicate urine or cell free liquid samples (plasma, serum, cell culture supernatant, etc…).

Intended Use: Troponin I M ELISA is a direct ELISA for quantitation of
murine Troponin I found in serum, plasma and supernatant. It is for
research purposes, and is not intended for diagnostic use.

Kit Contents: The Troponin I M ELISA kit contains the following items:
a. 1 Troponin I Assay Plate
b. 2 NHEBSA Diluent
c. 2 Troponin I M Standard
d. 1 Anti-Troponin I M antibody
e. 1 HRP-conjugated antibody
f. 1 Color Developer
g. 1 Color Stopper
h. 1 Instructions

Technical Background:

Troponin is a complex of three regulatory proteins that control actin and myosin interactions: C (calcium binding), T (tropomyosin binding), and I (inhibitory). This assay is a direct ELISA using two different affinity purified antibodies to mouse troponin I. Antibodies specific for cardiac troponin I do not react immunologically with the Troponin C or T.

A microtiter plate comes coated with the first antibody to which samples are added and incubated overnight. The plate then gets washed before the second antibody is added for a 2 hour room temperature incubation followed by colorimetric measurement. Given the high prevalence of coronary artery disease in patients with chronic kidney disease, cardiac troponins may be useful in predicting cardiovascular risk and mortality in patients with CKD.

Specimen Collection and Storage: Collect samples without preservative,
separate and clarify them by centrifugation if necessary. Samples
may be stored at 4˚C for up to 1 week or at -60˚C for up to 2 months. Prior to
assay, allow the samples to come to room temperature. Do not apply heat
to thaw frozen samples.

Specimen Required:  Urine, Serum, Plasma or tissue extract.

Assay Range: 0.78 – 50.0 ng/ml

Precision: Intra- and inter-assay precision for samples within the assay
range have a C.V. of <10%.


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