hHbA0 is a non-glycated fraction of human hemoglobin A0 prepared from lysed human blood cells by sequential ion-exchange and affinity chromatographic methods. It has less than 0.05 glyco groups per hemoglobin molecule, and is free of HbA1a, HbA1b HbA1c, HbF and glycated hemoglobin species. The protein is validated by HPLC methods, and is suitable as a primary standard in chromatographic or immunologic analyses. The reported concentration is determined by Drabkins assay.
The material is supplied in liquid form in dilute potassium cyanide-phosphate buffer. The concentration varies with manufacturing lot, but the protein may be used directly by dilution in buffer in most experimental methods. It is shipped frozen at -20oC.
Short term storage should be done at 4oC. Long term storage should be completed at -60oC. Repeated cycles of freezing and thawing should be avoided.
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