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Ensure consistent studies of diabetic nephropathy and other disease states where measurement of the inflammation biomarker CRP provides critical information.
FOR RESEARCH USE ONLY
Our Exocell CRP ELISA Kits support a range of studies with high-quality measurement of CRP levels in serum, plasma, or cell culture supernatants. Available for samples from rat, mouse, or humans, these kits are configured as quantitative sandwich ELISAs that use biotinylated detection antibodies. Measurement occurs through streptavidin-conjugated horse radish peroxidase (HRP) which binds to the biotinylated antibody, and a colorimetric HRP substrate that absorbs at 450 nm. Color intensity in all CRP ELISAs is proportional to the amount of CRP captured on the plate.
A widely-used biomarker for inflammation, measurement of blood CRP levels can provide a more nuanced view of diabetic nephropathy and other disease states when evaluated in parallel with other biomarkers, such as urinary albumin. Ensure the quality and consistency of your results by choosing an ELISA prepared by the same team of scientists that make your other Exocell products.
As with all of the Exocell ELISA products, our CRP ELISA Kits deliver the same reliable results and expert support that Exocell customers know to expect, including reagents that are ready-to-use either as is or with only moderate dilution.
What’s a sandwich ELISA?
In a sandwich ELISA, antigen measurement occurs by building an antibody-antigen-antibody “sandwich.” First, antibody that recognizes the target antigen is immobilized in the wells of a multi-well plate. The sample is added to the plate and target antigen binds to the immobilized antibody. After washing, a solution containing soluble antibody that recognizes the target antigen is added to detect and measure the amount of target antigen present in the wells.
To make a sandwich ELISA quantitative, it is critical to achieve uniform and consistent coating of the initial antibody to the multi-well plate.
Why use a sandwich ELISA?
Sandwich ELISAs are useful when purified target antigen is not available to create a competitive ELISA plate or when antibody to the target antigen is plentiful.
What is direct vs. indirect detection?
Direct versus indirect detection refers to how HRP is introduced into the assay. With direct detection, HRP is conjugated directly to the antibody that recognizes the analyte. This is the simpler and quicker of the two methods as only a single antibody is needed. With indirect detection, a primary antibody recognizes the analyte, and then a secondary antibody conjugated to HRP recognizes the primary antibody. The addition of the secondary antibody adds wash steps, increasing the time and labor involved in the assay workflow as well as adding steps where variability can be introduced.
CRP is a major positive acute-phase protein—i.e. CRP concentrations increase as part of the inflammation response—and is also an important biomarker for a number of age-related diseases such as cardiovascular disease, hypertension, diabetes mellitus, kidney disease, Alzheimer’s disease, and Parkinson’s disease1 as well as urologic cancers2. In mouse models, CRP is not just correlated with disease but also directly mediates kidney injury via TGF-β/Smad3 signaling pathways. And in clinical studies of diabetic patients, high sensitivity-CRP can provide additional insight into development of diabetic nephropathy3,4.
CRP consists of five identical 21.5 kDa subunits and is synthesized primarily in liver hepatocytes.