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Albuwell M (Mouse Albumin ELISA)

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Albuwell M: A murine microalbuminuria ELISA.

Intended Use: Albuwell M is an enzyme linked immunosorbent assay (ELISA) for the quantitative determination of albumin in mouse urine.

Kit Contents: Your Albuwell M kit should contain the following items:
1. 2 Albuwell M Test Plates
2. 2 NHEBSA (Diluent)
3. Mouse Serum Albumin (MSA) Standard
4. 2 Anti-mouse Albumin Ab-HRP Conjugate
5. 2 Color Developer
6. 2 Color Stopper
7. Instructions

Technical Background: Albuwell M is an in-vitro tool for assessing kidney function in mice. It is simple to perform and highly specific for mouse albumin. It is a competitive antibody capture ELISA completed in a direct mode. To that end, the anti-albumin antibody is conjugated to horseradish peroxidase (anti-mosue Albumin Ab-HRP), i.e. directly labeled.

To complete the assay, sample and anti-mouse albumin Ab-HRP are added to mouse albumin-coated well. The antibody interacts and binds with the albumin immobilized onto the stationary phase or with that in the fluid phase, hence the notion of competitive binding. Washing removes unbound Ab-HRP-Albumin and other reactants of the fluid phase from the well. Only the antibody-conjugate that was bound to the albumin of the stationary phase remains, and this is detected using Tetramethylbenzidine (TMB) in a chromogenic reaction. The reaction is stopped with acid, and absorbance is measured at 450 nm. Absorbance is inversely proportional to the logarithm of albumin concentration in the fluid phase.

Specimen Collection and Storage: Collect samples without preservative, and clarify them by centrifugation if necessary. Store clarified urine at 4oC for up to 1 week or at -60oC for up to 2 months. Prior to assay, allow the samples to come to room temperature. Do not apply heat to thaw frozen samples.

MSA Standard, NHEBSA and anti-mouse Albumin Ab-HRP Conjugate preparations contain 0.05% Proclin 300 (active components isothiazolones) as preservative. Color Stopper contains dilute (2.0 N) sulfuric acid. Albuwell M plates are precoated and ready to use. All kit reagents are supplied in ready to use liquid form.

Citations

Journal Title
Nature Blocking VEGFR-3 suppresses angiogenic sprouting and vascular network formation
Nat. Med. Pyruvate kinase M2 activation may protect against the progression of diabetic glomerular pathology and mitochondrial dysfunction
Nat. Med. Transgenic expression of human APOL1 risk variants in podocytes induces kidney disease in mice
Nat. Med. Tracking the fate of glomerular epithelial cells in vivo using serial multiphoton imaging in new mouse models with fluorescent lineage tags
Nat. Med. Angiotensin receptor agonistic autoantibodies induce pre-eclampsia in pregnant mice
Nat. Genet. Common variation in GPC5 is associated with acquired nephrotic syndrome
J. Am. Coll. Cardiol. Cardiovascular and systemic microvascular effects of anti-vascular endothelial growth factor therapy for cancer
Sci Transl Med Arginase-1-dependent promotion of TH17 differentiation and disease progression by MDSCs in systemic lupus erythematosus
Hepatology Human liver cell spheroids in extended perfusion bioreactor culture for repeated-dose drug testing
Am. J. Respir. Crit. Care Med. Segmental antigen challenge increases fibronectin in bronchoalveolar lavage fluid

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